10x chromium single cell immune profiling

#10x chromium single cell immune profiling full#

Vendor’s specification recommends getting at least 20,000 paired-end raw reads per cell for gene expression studies*. In order to read the transcript sequences on one end, and the barcode and UMI on the other end, paired-end sequencing reads are required. Libraries generated are compatible with Illumina sequencers. Transcripts from the same cell will be identified by a 10X barcode, while each of these transcripts will be discernible from one another by a Unique Molecular Identifier (UMI). Gene Expression Assays are able to capture 500 to 10,000 cells per sample from a single cell suspension.Ĭells will be encapsulated into Gel Beads-in-emulsion (GEMs) by the 10X Chromium Single Cell Controller, followed by reverse transcription and library preparation to become a pool of cDNA libraries. Single Cell Encapsulation and Library Preparation Please submit service request through iLab. Please refer to Sample Preparation and Submission section for sample requirements. Chromium Single Cell Immune Profiling provides a comprehensive approach to simultaneously examine cellular heterogeneity of the immune system, T- and B-cell. Affiliated institute (HKU, local academics, overseas academics, etc.).Number of target cells (affecting the sequencing cost).box in the top left corner of the browser. To identify the phenotype of the top T cell clonotypes, select Cell Types from the Split on Category. There are several ways to do this in Loupe Browser. CellBank Australia can prepare your cell samples for the single-cell 10X Chromium service. One key benefit of running an integrated 5' Gene Expression and V(D)J experiment is the ability to associate phenotypes with clonotypes of interest. 3′ gene expression, 5′ gene expression, V(D)J analysis, etc.) Single Cell Immune Profiling 5 v1.1 Single Index and v2 Dual. Please contact Dr KWOK, Hin ( 2831-5483) for project discussion and service quotation. The Chromium 10x allows for a variety of applications, including single cell gene expression, single cell immune profiling, and genome sequencing. Chromium Single Cell Immune Profiling provides a comprehensive approach to simultaneously examine cellular heterogeneity of the immune system, T- and B-cell repertoire diversity, and antigen specificity at single cell resolution.

#10x chromium single cell immune profiling full#

The Core offers full service from cell encapsulation using 10X Chromium Single Cell Controller, subsequent library preparation, Illumina sequencing to data analysis by Cell Ranger.

For non-working hours usage (after 17:30 on weekdays, whole days on weekends).

Enable multiomic profiling by measuring cell surface protein expression or CRISPR perturbations simultaneously from the same cell.

Able to examine gene expression profile and resolve TCR/BCR repertoires from the same sampleĪdd-on Feature Barcode technology with Single Cell Gene Expression Assays.

Assemble, annotate full length T cell and/or B cell V(D)J sequences and call clonotypes.

5′-end gene expression profiling of 500 – 10,000 cells per sample.

Single Cell 5′ Gene Expression & Immune Profiling 3′-end gene expression profiling of 500 – 10,000 cells per sample.Analyze full-length, paired B-cell or T-cell receptors. HKUMed Laboratory of Cellular Therapeutics Chromium Single Cell Immune Profiling provides a multiomic solution to your immunology questions.iScan Genotyping and Methylation Analysis Input: Labeled single cells in suspension + 10x Gel Beads and Reagents Output: Full-length paired V(D)J sequences, digital.

Join us for this webinar to hear about our latest advances in Targeted Gene Expression, Chromium Single Cell Immune Profiling v2 with Feature Barcode Technology, and the Visium Spatial Gene Expression Solution with Immunofluorescence. 14, 2023 /PRNewswire/ - 10x Genomics, Inc. At 10x Genomics, we provide single cell and spatial solutions that enable you to drive the leading edge of what’s possible. Speaker: Science & Technology Advisor, Leo Chan, Ph.D.,Īs we enter a century where transformative advances will reshape the way we examine biological complexities from single cells and tissues,researchers need approaches that can keep up with the fast pace of their science.Title: Latest Advances in 10x Genomics’ Single Cell and VisiumSpatial Gene Expression Solutions.This will be a precious opportunity for you to learn the latest advances in Targeted Gene Expression, Chromium Single Cell Immune Profiling v2 with Feature Barcode Technology, and the Visium Spatial Gene Expression Solution with Immunofluorescence. He will introduce Latest Advances in 10x Genomics. DNA sequencing section would like to invite you to the following seminar by Dr.Leo Chan.